Retroviral vectors pseudotyped with severe acute respiratory syndrome coronavirus S protein
Identifieur interne : 005A75 ( Main/Exploration ); précédent : 005A74; suivant : 005A76Retroviral vectors pseudotyped with severe acute respiratory syndrome coronavirus S protein
Auteurs : Tsanan Giroglou [Allemagne] ; Jindrich Jr Cinatl [Allemagne] ; Holger Rabenau [Allemagne] ; Christian Drosten [Allemagne] ; Harald Schwalbe [Allemagne] ; Hans Wilhelm Doerr [Allemagne] ; Dorothee Von Laer [Allemagne]Source :
- Journal of virology [ 0022-538X ] ; 2004.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antiviraux (immunologie), Cellules cultivées, Chats, Chiens, Délétion de séquence, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (immunologie), Glycoprotéines membranaires (métabolisme), Génie génétique, Humains, Lignée cellulaire tumorale, Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (immunologie), Protéines de l'enveloppe virale (métabolisme), Spécificité d'espèce, Spécificité d'organe, Suidae (virologie), Syndrome respiratoire aigu sévère (immunologie), Syndrome respiratoire aigu sévère (transmission), Syndrome respiratoire aigu sévère (virologie), Sérums immuns (immunologie), Tests de neutralisation, Vecteurs génétiques (génétique), Vecteurs génétiques (physiologie), Virus de la leucémie murine (génétique), Virus de la leucémie murine (immunologie), Virus de la leucémie murine (physiologie), Virus du SRAS (), Virus du SRAS (génétique), Virus du SRAS (immunologie), Virus du SRAS (physiologie), Zoonoses (transmission), Zoonoses (virologie).
- MESH :
- génétique : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Vecteurs génétiques, Virus de la leucémie murine, Virus du SRAS.
- immunologie : Anticorps antiviraux, Glycoprotéines membranaires, Protéines de l'enveloppe virale, Syndrome respiratoire aigu sévère, Sérums immuns, Virus de la leucémie murine, Virus du SRAS.
- métabolisme : Glycoprotéines membranaires, Protéines de l'enveloppe virale.
- physiologie : Vecteurs génétiques, Virus de la leucémie murine, Virus du SRAS.
- virologie : Suidae, Syndrome respiratoire aigu sévère, Zoonoses.
- Pascal (Inist)
- Animaux, Cellules cultivées, Chats, Chiens, Coronavirus, Délétion de séquence, Glycoprotéine de spicule des coronavirus, Génie génétique, Humains, Lignée cellulaire tumorale, Spécificité d'espèce, Spécificité d'organe, Syndrome respiratoire aigu sévère, Tests de neutralisation, Vecteur, Grave, Malade état grave, Aigu, Virus du SRAS, Voie respiratoire, Vitronectine, Protéine, Microbiologie, Syndrome respiratoire aigu sévère, Virologie, Forme grave, Zoonoses.
English descriptors
- KwdEn :
- Acute, Animals, Antibodies, Viral (immunology), Cats, Cell Line, Tumor, Cells, Cultured, Coronavirus, Critically ill, Dogs, Genetic Engineering, Genetic Vectors (genetics), Genetic Vectors (physiology), Humans, Immune Sera (immunology), Leukemia Virus, Murine (genetics), Leukemia Virus, Murine (immunology), Leukemia Virus, Murine (physiology), Membrane Glycoproteins (genetics), Membrane Glycoproteins (immunology), Membrane Glycoproteins (metabolism), Microbiology, Neutralization Tests, Organ Specificity, Protein, Respiratory tract, SARS Virus (classification), SARS Virus (genetics), SARS Virus (immunology), SARS Virus (physiology), Sequence Deletion, Severe, Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (transmission), Severe Acute Respiratory Syndrome (virology), Severe acute respiratory syndrome, Species Specificity, Spike Glycoprotein, Coronavirus, Swine (virology), Vector, Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology), Viral Envelope Proteins (metabolism), Virology, Vitronectin, Zoonoses (transmission), Zoonoses (virology).
- MESH :
- chemical , genetics : Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , immunology : Antibodies, Viral, Immune Sera, Membrane Glycoproteins, Viral Envelope Proteins.
- classification : SARS Virus.
- genetics : Genetic Vectors, Leukemia Virus, Murine, SARS Virus.
- immunology : Leukemia Virus, Murine, SARS Virus, Severe Acute Respiratory Syndrome.
- chemical , metabolism : Membrane Glycoproteins, Viral Envelope Proteins.
- physiology : Genetic Vectors, Leukemia Virus, Murine, SARS Virus.
- transmission : Severe Acute Respiratory Syndrome, Zoonoses.
- virology : Severe Acute Respiratory Syndrome, Swine, Zoonoses.
- Animals, Cats, Cell Line, Tumor, Cells, Cultured, Dogs, Genetic Engineering, Humans, Neutralization Tests, Organ Specificity, Sequence Deletion, Species Specificity, Spike Glycoprotein, Coronavirus.
Abstract
The worldwide outbreak of severe acute respiratory syndrome (SARS) was shown to be associated with a novel coronavirus (CoV) now called SARS CoV. We report here the generation of SARS CoV S protein-pseudotyped murine leukemia virus (MLV) vector particles. The wild-type S protein pseudotyped MLV vectors, although at a low efficiency. Partial deletion of the cytoplasmic tail of S dramatically increased infectivity of pseudotypes, with titers only two- to threefold lower than those of pseudotypes generated in parallel with the vesicular stomatitis virus G protein. S-pseudotyped MLV particles were used to analyze viral tropism. MLV (SARS) pseudotypes and wild-type SARS CoV displayed similar cell types and tissue and host restrictions, indicating that the expression of a functional receptor is the major restraint in permissiveness to SARS CoV infection. Efficient gene transfer could be detected in Vero and CaCo2 cells, whereas the level of gene marking of 293T, HeLa, and HepG2 cells was only slightly above background levels. A cat cell line and a dog cell line were not susceptible. Interestingly, PK-15, a porcine kidney cell line, and primary porcine kidney cells were also highly permissive for SARS S pseudotypes and wild-type SARS CoV. This finding suggests that swine may be susceptible to SARS infection and may be a source for infection of humans. Taken together, these results indicate that MLV(SARS) pseudotypes are highly valuable for functional studies of viral tropism and entry and, in addition, can be a powerful tool for the development of therapeutic entry inhibitors without posing a biohazard to human beings.
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Le document en format XML
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Rabenau</name><affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Institute of Medical Virology, Frankfurt University Medical School</s1><s2>Frankfurt</s2><s3>DEU</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Allemagne</country><placeName><region type="land" nuts="1">Hesse (Land)</region><region type="district" nuts="2">District de Darmstadt</region><settlement type="city">Francfort-sur-le-Main</settlement></placeName></affiliation></author><author><name sortKey="Drosten, Christian" sort="Drosten, Christian" uniqKey="Drosten C" first="Christian" last="Drosten">Christian Drosten</name><affiliation wicri:level="3"><inist:fA14 i1="03"><s1>Institut für Organische Chemie, Johann Wolfgang Goethe Universität</s1><s2>Frankfurt</s2><s3>DEU</s3><sZ>4 aut.</sZ></inist:fA14><country>Allemagne</country><placeName><region type="land" nuts="1">Hesse (Land)</region><region type="district" nuts="2">District de Darmstadt</region><settlement type="city">Francfort-sur-le-Main</settlement></placeName></affiliation></author><author><name sortKey="Schwalbe, Harald" sort="Schwalbe, Harald" uniqKey="Schwalbe H" first="Harald" last="Schwalbe">Harald Schwalbe</name><affiliation wicri:level="3"><inist:fA14 i1="04"><s1>Department of Virology, Bernhard Nocht Institute for Tropical Medicine</s1><s2>Hamburg</s2><s3>DEU</s3><sZ>5 aut.</sZ></inist:fA14><country>Allemagne</country><placeName><settlement type="city">Hambourg</settlement><region type="land" nuts="2">Hambourg</region></placeName></affiliation></author><author><name sortKey="Doerr, Hans Wilhelm" sort="Doerr, Hans Wilhelm" uniqKey="Doerr H" first="Hans Wilhelm" last="Doerr">Hans Wilhelm Doerr</name><affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Institute of Medical Virology, Frankfurt University Medical School</s1><s2>Frankfurt</s2><s3>DEU</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Allemagne</country><placeName><region type="land" nuts="1">Hesse (Land)</region><region type="district" nuts="2">District de Darmstadt</region><settlement type="city">Francfort-sur-le-Main</settlement></placeName></affiliation></author><author><name sortKey="Von Laer, Dorothee" sort="Von Laer, Dorothee" uniqKey="Von Laer D" first="Dorothee" last="Von Laer">Dorothee Von Laer</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Georg-Speyer-Haus, Institute for Biomedical Research</s1><s2>Frankfurt</s2><s3>DEU</s3><sZ>1 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Allemagne</country><placeName><region type="land" nuts="1">Hesse (Land)</region><region type="district" nuts="2">District de Darmstadt</region><settlement type="city">Francfort-sur-le-Main</settlement></placeName></affiliation></author></analytic><series><title level="j" type="main">Journal of virology</title><title level="j" type="abbreviated">J. virol.</title><idno type="ISSN">0022-538X</idno><imprint><date when="2004">2004</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of virology</title><title level="j" type="abbreviated">J. virol.</title><idno type="ISSN">0022-538X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acute</term><term>Animals</term><term>Antibodies, Viral (immunology)</term><term>Cats</term><term>Cell Line, Tumor</term><term>Cells, Cultured</term><term>Coronavirus</term><term>Critically ill</term><term>Dogs</term><term>Genetic Engineering</term><term>Genetic Vectors (genetics)</term><term>Genetic Vectors (physiology)</term><term>Humans</term><term>Immune Sera (immunology)</term><term>Leukemia Virus, Murine (genetics)</term><term>Leukemia Virus, Murine (immunology)</term><term>Leukemia Virus, Murine (physiology)</term><term>Membrane Glycoproteins (genetics)</term><term>Membrane Glycoproteins (immunology)</term><term>Membrane Glycoproteins (metabolism)</term><term>Microbiology</term><term>Neutralization Tests</term><term>Organ Specificity</term><term>Protein</term><term>Respiratory tract</term><term>SARS Virus (classification)</term><term>SARS Virus (genetics)</term><term>SARS Virus (immunology)</term><term>SARS Virus (physiology)</term><term>Sequence Deletion</term><term>Severe</term><term>Severe Acute Respiratory Syndrome (immunology)</term><term>Severe Acute Respiratory Syndrome (transmission)</term><term>Severe Acute Respiratory Syndrome (virology)</term><term>Severe acute respiratory syndrome</term><term>Species Specificity</term><term>Spike Glycoprotein, Coronavirus</term><term>Swine (virology)</term><term>Vector</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Envelope Proteins (immunology)</term><term>Viral Envelope Proteins (metabolism)</term><term>Virology</term><term>Vitronectin</term><term>Zoonoses (transmission)</term><term>Zoonoses (virology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps antiviraux (immunologie)</term><term>Cellules cultivées</term><term>Chats</term><term>Chiens</term><term>Délétion de séquence</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (génétique)</term><term>Glycoprotéines membranaires (immunologie)</term><term>Glycoprotéines membranaires (métabolisme)</term><term>Génie génétique</term><term>Humains</term><term>Lignée cellulaire tumorale</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Protéines de l'enveloppe virale (immunologie)</term><term>Protéines de l'enveloppe virale (métabolisme)</term><term>Spécificité d'espèce</term><term>Spécificité d'organe</term><term>Suidae (virologie)</term><term>Syndrome respiratoire aigu sévère (immunologie)</term><term>Syndrome respiratoire aigu sévère (transmission)</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Sérums immuns (immunologie)</term><term>Tests de neutralisation</term><term>Vecteurs génétiques (génétique)</term><term>Vecteurs génétiques (physiologie)</term><term>Virus de la leucémie murine (génétique)</term><term>Virus de la leucémie murine (immunologie)</term><term>Virus de la leucémie murine (physiologie)</term><term>Virus du SRAS ()</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (immunologie)</term><term>Virus du SRAS (physiologie)</term><term>Zoonoses (transmission)</term><term>Zoonoses (virologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Viral</term><term>Immune Sera</term><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Genetic Vectors</term><term>Leukemia Virus, Murine</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Vecteurs génétiques</term><term>Virus de la leucémie murine</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antiviraux</term><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Syndrome respiratoire aigu sévère</term><term>Sérums immuns</term><term>Virus de la leucémie murine</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Leukemia Virus, Murine</term><term>SARS Virus</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Vecteurs génétiques</term><term>Virus de la leucémie murine</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Genetic Vectors</term><term>Leukemia Virus, Murine</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="transmission" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term><term>Zoonoses</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Suidae</term><term>Syndrome respiratoire aigu sévère</term><term>Zoonoses</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term><term>Swine</term><term>Zoonoses</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cats</term><term>Cell Line, Tumor</term><term>Cells, Cultured</term><term>Dogs</term><term>Genetic Engineering</term><term>Humans</term><term>Neutralization Tests</term><term>Organ Specificity</term><term>Sequence Deletion</term><term>Species Specificity</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Animaux</term><term>Cellules cultivées</term><term>Chats</term><term>Chiens</term><term>Coronavirus</term><term>Délétion de séquence</term><term>Glycoprotéine de spicule des coronavirus</term><term>Génie génétique</term><term>Humains</term><term>Lignée cellulaire tumorale</term><term>Spécificité d'espèce</term><term>Spécificité d'organe</term><term>Syndrome respiratoire aigu sévère</term><term>Tests de neutralisation</term><term>Vecteur</term><term>Grave</term><term>Malade état grave</term><term>Aigu</term><term>Virus du SRAS</term><term>Voie respiratoire</term><term>Vitronectine</term><term>Protéine</term><term>Microbiologie</term><term>Syndrome respiratoire aigu sévère</term><term>Virologie</term><term>Forme grave</term><term>Zoonoses</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The worldwide outbreak of severe acute respiratory syndrome (SARS) was shown to be associated with a novel coronavirus (CoV) now called SARS CoV. We report here the generation of SARS CoV S protein-pseudotyped murine leukemia virus (MLV) vector particles. The wild-type S protein pseudotyped MLV vectors, although at a low efficiency. Partial deletion of the cytoplasmic tail of S dramatically increased infectivity of pseudotypes, with titers only two- to threefold lower than those of pseudotypes generated in parallel with the vesicular stomatitis virus G protein. S-pseudotyped MLV particles were used to analyze viral tropism. MLV (SARS) pseudotypes and wild-type SARS CoV displayed similar cell types and tissue and host restrictions, indicating that the expression of a functional receptor is the major restraint in permissiveness to SARS CoV infection. Efficient gene transfer could be detected in Vero and CaCo2 cells, whereas the level of gene marking of 293T, HeLa, and HepG2 cells was only slightly above background levels. A cat cell line and a dog cell line were not susceptible. Interestingly, PK-15, a porcine kidney cell line, and primary porcine kidney cells were also highly permissive for SARS S pseudotypes and wild-type SARS CoV. This finding suggests that swine may be susceptible to SARS infection and may be a source for infection of humans. Taken together, these results indicate that MLV(SARS) pseudotypes are highly valuable for functional studies of viral tropism and entry and, in addition, can be a powerful tool for the development of therapeutic entry inhibitors without posing a biohazard to human beings.</div></front></TEI><affiliations><list><country><li>Allemagne</li></country><region><li>District de Darmstadt</li><li>Hambourg</li><li>Hesse (Land)</li></region><settlement><li>Francfort-sur-le-Main</li><li>Hambourg</li></settlement></list><tree><country name="Allemagne"><region name="Hesse (Land)"><name sortKey="Giroglou, Tsanan" sort="Giroglou, Tsanan" uniqKey="Giroglou T" first="Tsanan" last="Giroglou">Tsanan Giroglou</name></region><name sortKey="Cinatl, Jindrich Jr" sort="Cinatl, Jindrich Jr" uniqKey="Cinatl J" first="Jindrich Jr" last="Cinatl">Jindrich Jr Cinatl</name><name sortKey="Doerr, Hans Wilhelm" sort="Doerr, Hans Wilhelm" uniqKey="Doerr H" first="Hans Wilhelm" last="Doerr">Hans Wilhelm Doerr</name><name sortKey="Drosten, Christian" sort="Drosten, Christian" uniqKey="Drosten C" first="Christian" last="Drosten">Christian Drosten</name><name sortKey="Rabenau, Holger" sort="Rabenau, Holger" uniqKey="Rabenau H" first="Holger" last="Rabenau">Holger Rabenau</name><name sortKey="Schwalbe, Harald" sort="Schwalbe, Harald" uniqKey="Schwalbe H" first="Harald" last="Schwalbe">Harald Schwalbe</name><name sortKey="Von Laer, Dorothee" sort="Von Laer, Dorothee" uniqKey="Von Laer D" first="Dorothee" last="Von Laer">Dorothee Von Laer</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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